A: Infrared Absorption á197Kñ.

B: Ultraviolet Absorption á197Uñ—

Solution: 80µg per mL.

Medium: 0.1Nhydrochloric acid.

Standard solution— Dissolve an accurately weighed quantity of USP Albuterol RSin methanol to obtain a solution having a known concentration of 0.10mg per mL.

Test solution— Dissolve an accurately weighed quantity of Albuterol in methanol to obtain a solution having a concentration of 20mg per mL.

Procedure— Apply 10-µLaliquots of the Test and the Standard solutionto separate points on a thin-layer chromatographic plate (see Chromatography á621ñ)coated with a 0.25-mm layer of chromatographic silica gel.Place the plate in a saturated chromatographic chamber,and develop the chromatogram in a solvent system consisting of a mixture of methyl isobutyl ketone,isopropyl alcohol,ethyl acetate,water,and ammonium hydroxide (50:45:35:18:3)until the solvent front has moved three-fourths of the length of the plate.Remove the plate from the developing chamber,air-dry,and expose it to iodine vapor:any spot,other than the principal spot,obtained from the Test solutionis not greater in size and intensity than the spot produced by the Standard solution(0.5%),and the sum of the impurities is not greater than 2.0%.