Related compounds
[NOTECarry out the operations avoiding exposure to actinic light. All materials that are in direct contact with Ciclopirox Olamine (e.g., column materials, reagents, solvents, etc.) should contain only very low amounts of extractable metal cations.
]
Mobile phase
Prepare a filtered and degassed mixture of an edetate disodium solution (0.96 in 1000), acetonitrile, and glacial acetic acid (770:230:0.1). Make adjustments if necessary (see
System Suitability under
Chromatography 621).
Rinsing solution
Prepare a mixture of water, acetonitrile, glacial acetic acid, and acetylacetone (500:500:1:1).
Standard stock solution
Dissolve 15 mg of USP Ciclopirox Related Compound A RS and 15 mg of USP Ciclopirox Related Compound B RS, accurately weighed, in 1 mL of acetonitrile and 7 mL of Mobile phase. Dilute the solution thus obtained with Mobile phase to 10.0 mL to obtain a solution having a known concentration of 1.5 mg of each USP Reference Standard per mL.
Standard solution A
Dilute 1.0 mL of Standard stock solution to 200.0 mL with a mixture of Mobile phase and acetonitrile (9:1).
Standard solution B
Dilute 2.0 mL of Standard solution A to 10.0 mL with a mixture of Mobile phase and acetonitrile (9:1).
Test solution
Dissolve 40 mg of Ciclopirox Olamine, accurately weighed, in a mixture of 2 mL of acetonitrile, 20 µL of glacial acetic acid, and 15 mL of Mobile phase. If necessary, use an ultrasonic bath to dissolve. Dilute with Mobile phase to 20.0 mL, and mix.
Resolution solution
Mix 5 mL of Standard stock solution with 5 mL of the Test solution.
Chromatographic system (see Chromatography 621)
The liquid chromatograph is equipped with a detector capable of recording at both 220 nm and 298 nm and a 4.0-mm × 8-cm column that contains packing L10.
[NOTECiclopirox related compound A has an intense absorbance at 220 nm, and 6-cyclohexyl-4-methyl-2(1
H)-pyridone, ciclopirox related compound B, and ciclopirox have intense absorbances at 298 nm.
] The flow rate is about 0.7 mL per minute. Chromatograph the
Resolution solution at 298 nm, and record the peak responses as directed for
Procedure: the resolution,
R, between the ciclopirox related compound B peak and the ciclopirox peak is not less than 2.0. Chromatograph
Standard solution B at 298 nm, and record the peak responses as directed for
Procedure: the chromatogram obtained shows at 298 nm a peak corresponding to ciclopirox related compound B with a signal-to-noise ratio of not less than 3. Chromatograph the
Test solution at 298 nm, and record the peak responses as directed for
Procedure: the tailing factor for the ciclopirox peak is less than 2.0.
Procedure
Separately inject equal volumes (about 10 µL) of Standard solution A, Standard solution B, and the Test solution into the chromatograph, and record the chromatograms. [NOTEIn order to ensure desorption of disruptive metal ions, every new column must be rinsed with the Rinsing solution over a period of not less than 15 hours and then with Mobile phase for not less than 5 hours with a flow rate of 0.2 mL per minute. The chromatographic run time is not less than 2.5 times the retention time of the ciclopirox peak.] The relative retention times are about 0.5 for ciclopirox related compound A, 0.9 for 6-cyclohexyl-4-methyl-2(1H)-pyridone, 1.0 for ciclopirox, and 1.3 for ciclopirox related compound B. The peak response at 220 nm of the ciclopirox related compound A peak in the chromatogram obtained from the Test solution is not more than the peak response at 220 nm of the corresponding peak in the chromatogram obtained from Standard solution A (0.5% with reference to ciclopirox). The sum of responses at 298 nm of the impurity peaks in the chromatogram obtained from the Test solution is not more than the peak response at 298 nm of the ciclopirox related compound B peak in the chromatogram obtained from Standard solution A (0.5% with reference to ciclopirox). At 298 nm disregard any peak due to the solvent and any peak with a response less than the response of the ciclopirox related compound B peak in the chromatogram obtained from Standard solution B at 298 nm (0.1% with reference to ciclopirox).