Procedure
To each of the flasks containing the
Assay preparation and the
Standard preparation, and to a similar flask containing 20.0 mL of alcohol, to provide the blank, add 2.0 mL of a solution prepared by dissolving 50 mg of blue tetrazolium in 10 mL of alcohol, and mix. Then to each flask add 4.0 mL of a mixture of 1 volume of
tetramethylammonium hydroxide TS and 9 volumes of alcohol. Mix, allow to stand in the dark for 90 minutes, add 1.0 mL of glacial acetic acid, mix, and proceed as directed for
Procedure under
Assay for Steroids 351, beginning with Concomitantly determine the absorbances. Calculate the quantity, in mg, of C
26H
33NaO
8 in the portion of Methylprednisolone Sodium Succinate taken by the formula:
8.37C(AU / AS).