Procedure
Proceed as directed for
Procedure under
Single-Steroid Assay 511, using a solvent system consisting of a mixture of
n-heptane and acetone (3:1), through the fourth sentence of the second paragraph under
Procedure. Then centrifuge the tubes for 5 minutes, and determine the absorbances of the supernatants in 1-cm cells at the wavelength of maximum absorbance at about 239 nm, with a suitable spectrophotometer, against the blank. Calculate the quantity, in mg, of C
27H
34O
3 in the portion of Nandrolone Phenpropionate taken by the formula:
10C(AU / AS),
in which
C is the concentration, in mg per mL, of
USP Nandrolone Phenpropionate RS in the
Standard preparation, and
AU and
AS are the absorbances of the solutions from the
Assay preparation and the
Standard preparation, respectively.